Oxidative Phosphorylation in Pea Cotyledon Submitochondrial Particles
نویسندگان
چکیده
منابع مشابه
Kinetics of ATP synthesis in pea cotyledon submitochondrial particles.
A kinetic study of oxidative phosphorylation by pea submitochondrial particles gave two K(m) values for ADP, one low, the other high. The high value probably reflected a damaged site or a population of leaky mitochondria. Only the high affinity site with a low K(m) for ADP was involved in ATP synthesis. alpha,beta-Methylene ADP was found to be a competitive inhibitor of ATP synthesis. The inorg...
متن کاملATPase Activity of Pea Cotyledon Submitochondrial Particles: ACTIVATION, SUBSTRATE SPECIFICITY, AND ANION EFFECTS.
Submitochondrial particles freshly prepared by sonication from pea cotyledon mitochondria showed low ATPase activity. Activity increased 20-fold on exposure to trypsin. The pea cotyledon submitochondrial particle ATPase was also activated by "aging" in vitro. At pH 7.0 addition of 1 millimolar ATP prevented the activation. ATPase of freshly prepared pea cotyledon submitochondrial particles had ...
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Addition of several N-arylaminonaphthalenesulfonate dyes to submitochondrial particles from bovine heart resulted in an enhancement of fluorescence of from SOto 150-fold and a considerable shift in the wave length of maximum emission toward the ultraviolet. The results indicate 40 to 55 dyebinding sites per mg of submitochondrial particles. A preliminary search revealed that mitochondrial ATPas...
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A protein designated phosphor!// Iransferasr has bee11 isolated from beef heart mitochondria and has been found to increase the ability of suhnlitocllorldrial particles, oxidizing either NASH or snecinatc, to synthesize ,4TP from ADI and inorganic orthophosphate. Increases in the value of P:O ratios between 0.45 and 1 have been observed wit,h srlccinate as snbstrat e. Phosphoryl trnnsferase was...
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High concentrations of uncouplers of oxidative phosphorylation inhibit the oxidation of succinate and NADH catalyzed by phosphorylating beef heart submitochondrial particles. Inhibition is reversed by bovine serum albumin but not by ATP. ATP does not influence either the stimulation of respiration at low uncoupler concentrations or the inhibition of respiration at high uncoupler concentrations....
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1979
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.64.5.757